Author(s):

R Kowsalya, A Vinoth and S Ramya

Abstract: Nanotechnology is important in the major fields of biology, chemistry, physics, and material sciences. Nanotechnology deals with the study of materials at the nanometers. The green synthesis of silver and Zinc nanoparticles using aqueous extract of <em>Enicostemma littorale </em>was also investigated <em>E. littorale</em> is a glabrous perennial herb belongs to family Gentianaceae. It possesses anti-oxidant, hypolipidemic, anti-microbial, anti-nociceptive, anti-edematologinic and anti-tumor activities. The UV-Vis spectrum of the prepared nanoparticles can be measured in the wavelength range of 200-700 nm, The Ultraviolet (UV) region scanned is normally from 200-400 nm and the visible portion is from 400-600 nm Complete removal of water has been confirmed by recording the FTIR spectrum of pure KBr, The synthesized Silver and Zinc nanoparticles by Characterization studies in Scanning Electron Microscope (SEM), The antibacterial activities of synthesized AgNPs and ZnONPs were carriedout by well diffusion methods. 5gm of <em>Enicostemma littorale</em> leaf powder, in dried form, was added into 100 mL of distilled water and magnetically stirred for 2 h at 80 °C. After cooling to room temperature and filtering through Whatman No. 1 paper, to remove particulate matter and to get clear solutions then refrigerated (4 °C) in 250 mL Erlenmeyer flasks for further experiments.10 mL of <em>Enicostemma littorale</em> plant leaf extract was mixed with 90 ml of 1 mM AgNO₃and Zinc aqueous solution in 250 mL conical flask. The reaction mixture was heated at 80 °C for one hour. The light yellowish brown coloured solution was changed into dark brown, which shows the reduction of Ag+ ions to metallic Ag. The present study systematically evaluated the antibacterial effect of <em>Enicostemma littoreale </em>nanoparticles<em> </em>against a Gram Positive and Gram negative bacteria. The Ag NPs and Zinc effectively inhibited the growth of both Gram Negative and Gram Positive bacteria. Our results show that Ag NPs and Zinc synthesized using an aqueous extract of <em>Enicostemma littorale</em> have potential antibacterial activity against both Gram Negative and Gram Positive bacteria.

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