Author(s):

Dharmaraj K, S Ezhilkumar, R Dinesh and R Ananadan

Abstract: Varietal identification of rice by ISSR markers allows precise, objective and rapid method. The materials for this study consisted of 10 rice genotypes viz. ADT 36, ADT 37, ADT 38, ADT 39, ADT 42, CO 42, CO 43, CO 45, CO 47 and ASD 20 were subjected to molecular characterization using Inter Simple Sequence Repeats (ISSRs) markers. In 10 rice genotypes, 15 ISSR primers were used to check polymorphism in which 11 primers were found to be polymorphic. The number of ISSR markers ranged from 3 as produced by primer UBC-857 and 44 as yielded by primer UBC-820 among the ten genotypes studied. Out of the total 292 ISSR markers amplified, only 209 markers were found to be polymorphic which had resulted 71.5 per cent polymorphism. UPGMA was performed using jaccard’s similarity coefficient matrices calculated from 15 ISSR markers to generate a dendrogram for 10 rice genotypes. The primer UBC-809 identified the rice genotypes ADT 36, ASD 20 and UBC-834 identified ADT 38 and CO 47 rice genotypes. The present study revealed that the ISSR primer UBC-834 identified two genotypes viz. ADT 38 and CO 47. The ISSR primer UBC-840 identified the genotypes ADT 3, CO 45 and UBC-841 identified the genotypes’ ADT 36 and UBC 879 identified the genotypes CO 47. The ISSR primer UBC-808 identified one genotype of rice ADT 42. The ISSR primer UBC 809 identified two genotypes ADT 36 and ASD 20. The ISSR primer UBC 834 identified two genotypes ADT 38 and CO 47. UBC-840 identified the genotypes ADT 39, CO 45 and UBC-841 identified the genotype ADT 36 and UBC-879 identified the genotype CO 45.

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