Author(s):

Bhavya Shree, Manigopa Chakraborty, Madhuparna Banerjee, Krishna Prasad, Rana Monalisha and Veena Kumari Tudu

Abstract: Maize (Zea mays L.) is the third most important cereal crop in the world. It is a major cereal crop for livestock feed, human nutrition and important raw material for several agro-based industries, there is great demand for maize of both quality and quantity. An efficient plant tissue culture procedure with high regeneration frequency is prerequisite for this approach. In light of the situation an experiment was conducted with an objective to optimize regeneration of plants using different explants of maize. The experiment was conducted using two hybrids (GS-802, 27P17) and one composite (BVM-2) for protocol development for in vitro regeneration of maize (Zea mays L.) using three different explants viz., shoots, endosperm, ovules. Shoots of all the genotypes were inoculated in MS media supplemented with 1mg/l BAP, 2mg/l BAP, 3mg/l BAP, 4mg/l BAP and 5mg/l BAP along with a control. Endosperm of all the genotypes were cultured in callusing media containing MS basal salts supplemented with 1mg/l 2, 4-D+1mg/l NAA and 0.2mg/l 2, 4-D+ 2mg/l NAA. Ovules were inoculated in the N6 medium. Better response of kernels regeneration was observed when treated with HgCl₂for 10 minutes. The response was observed for shoot elongation with 2mg/l BAP in GS-802. Highest shoot survival was observed in GS-802 with 2mg/l BAP and 4mg/l BAP. In endosperm culture, callusing observed on MS medium with different concentration of 2, 4-D+NAA. Better response of GS-802 was recorded in the hormonal combination of 2, 4-D-0.5mg/l + NAA-2mg/l and for BVM-2 in the hormonal combination of 2, 4-D-1mg/l + NAA-1mg/l. In case of ovule culture 85.71% of sprouting was observed in GS-802 genotype followed by BVM-2 and 27P17 with 76.91% and 47.61% respectively

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