Author(s):

Kiran Kachhap, Pallavi Sharma, Meena Misra, and Amarendra Narayan Misra

Abstract: Ocimum sanctum (L.) plant parts, especially the leaves contain secondary metabolites which are economically important due to its medicinal, culinary and aromatic properties. Callus culture is an alternative source to whole plants for the production of secondary metabolites. The objective of this study is to increase secondary metabolite production in Ocimum sanctum (L.) callus culture. Callus cultures were initiated on Murashige and Skoog (MS) medium supplemented with different concentrations (0.1-1.4 mg/l) of 2, 4-dichlorophenoxy acetic acid (2,4-D). The cultures were maintained under continuous illumination of white light (125 uμmole. m^-2.s^-1). Maximum callusing was observed in MS medium supplemented with 0.1 mg/l 2, 4-D and decreased at higher concentrations (0.2, 0.4, 0.6, 0.8, 1 mg/l). Initiation of callus was totally arrested when explants were grown in MS media supplemented with 1.2 or 1.4 mg/l of 2, 4-D.

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