Author(s):

Amol D Sable, Prashant B Kardile, Asmita D Sable and Ashok V Kharde

Abstract: Bacopa monnieri has been chosen as a model medicinal plant to study the Abiotic stress (salinity) under in vitro system. In the present investigation effect of incrasing concentration of NaCl on Bacoside production of Brahmi (Bacopa monnieri (L.) Pennell) was studies under in vitro condition. Nodal segments of Brahmi were inoculated on the MS media supplemented with BA(1.1µM) and IBA(0.30µM). The salt stress was given to these plants by incorporating increasing amount of NaCl (00, 05, 10, 15, 20, 25 and 30 mM) in seven different treatements with three replication. After 4 weeks of inoculation, Bacoside content (4.13 %), Number of shoots (5.33/ explant), were produced in the treatement without NaCl. The Bacoside content was produced up to (0.70 %) in 30 mM NaCl at 30 DAI. The overall growth is reduced in proportion to the increase in NaCl concentration. Brahmi tolerates salt stress up to two levels of NaCl concentration i.e. 05 and 10 mM with minimum reduction in Bacoside production up to 25 and 54% respectively above reduction i.e. up to 94 % in 30 mM NaCl.

Pages: 1386-1389  |  1409 Views  357 Downloads

Download (506KB)