Author(s):

Manikant Tripathi, Mohammed Haroon and Shailendra Kumar

Abstract: Uttar Pradesh is the largest state in the country. Diarrhoea is one of the major cause for death of young children below the age of 5 years. The aim of the present study was to study bacterial isolates from the diarrhoeal stool samples of pediatric patients and their antibiotic sensitivity assay. A total of 30 patients were screened for the symptoms of disease and sampling was performed. The sampling and isolation resulted in 18 Gram-negative rod shaped isolates and other Gram-positive cocci. The Gram-negative bacteria were subjected to biochemical identification and antibiotic sensitivity assay followed by plasmid DNA isolation and characterization. The isolates were identified biochemically as Escherichia coli. Further, the antibiotic sensitivity assay showed great resistance among the isolates for the commonly used antibiotics against diarrhoeal diseases. The plasmid study showed that the trait of antibiotic resistance is located on the plasmid as the plasmid curing study led to the development of sensitivity of selected isolates towards the antibiotics

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