Author(s):

Prerit Kumar Singh, RK Joshi and RP Diwakar

Abstract: Isolation and characterization of Methicillin resistant S. aureus was attempted from pigs, buffalos, goats and sheep and the antibiotic resistant pattern of S. aureus isolates was studied in the present study. Processing of 90 milk samples (buffaloes 30, goats 40 and sheep 20) yielded 121 S. aureus isolates. All isolates were catalase positive and majority of isolates gave positive slide caogulase and / or tube coagulase test, nitrate reduction and β haemolysis and fermented mannitol sugar. Among S. aureus isolates from pigs, highest isolates showed resistance to ampicillin followed by cephalexin, methicillin, lincomycin, chloramphenicol, streptomycin and tetracycline. All the isolates were found sensitive to gentamicin and ciprofloxacin. From buffaloes, highest isolates exhibited resistance towards ampicillin and cephalexin followed by chloramphenicol, lincomycin, methicillin, tetracycline, ciprofloxacin streptomycin. All the isolates were sensitive for gentamicin. From goats, resistance for ampicillin was revealed by highest isolates followed by chloramphenicol, lincomycin, tetracycline cephalexin, streptomycin, methicillin and only one isolate was resistant to gentamicin. All the isolates from goats were found sensitive to ciprofloxacin. Similarly in sheep, highest resistant isolates were found for cephalexin followed by chloramphenicol, ampicillin, lincomycin, streptomycin, methicillin and tetracycline respectively. All the isolates from sheep were found sensitive to gentamicin and ciprofloxacin.

Pages: 1056-1061  |  1286 Views  232 Downloads

Download (487KB)