Author(s):

Preeti Rajoriya, Vivek Kumar Singh, Nancy Jaiswal and Rohit Lall

Abstract: Red banana is an important fruit crop of India. The micro propagation of shoot tips in vitro is a widespread application of biotechnology in agriculture. Micro propagation is advantageous over conventional techniques traditional propagation, opens fresh avenues towards conservation of endangered species, disease free plants, rapid and large scale production of elite plants all year round. Therefore, the current investigation emphasizes the effect of plant growth regulators on the micro propagation as well as on the biochemical estimation of red banana. The meristem tip was used as explants. Among the tested combinations and concentrations 3 mg/l BAP + 0.2 mg/l NAA showed highest shooting and 2 mg/l IBA with 0.5 and 1 mg/l IAA also. The highest root length and no. of roots was observed with the 1 mg/l IBA. Subsequently, the micro propagated plants were hardened in green house followed by the acclimatization. Significant increase in chlorophyll, carbohydrate, protein and phenol content was observed. These findings concluded that a fruitful and practicable protocol was set up for the successful regeneration of new plantlets of red banana. Micro propagation of red banana is beneficial further in somaclonal variation, genetic transformation and secondary metabolite production. The nutritional and medicinal potential of red banana is well documented which can be practiced as an alternative remedy for treating certain ailments.

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