Author(s):

Ranju Kumari, AK Singh, VK Sharma and Manish Kumar

Abstract: Electrophoretic separation of isozymes has provided a reasonably precise and quantitative approach for the analysis of genetic diversity. Thus, in the present study isozymes markers was used for assessing the genetic diversity in eight inbred lines of maize. namely CML 142, CML 144, CML 150, CML 176, CML 186 CM 300, CM 400 and CM 600. The horizontal starch gel electrophoresis was used to study the isozyme patterns. These parental lines were obtained from AICRP on Maize, Dholi Centre under RAU, Pusa, and Bihar. Six isozyme systems, namely peroxidase, esterase, acid phosphatase, catalase, amylase and alcohol dehydrogenase were used for characterization and diversity studies of inbreds. Polymorphism of isozyme was visualized in respect of number and mobility of bands. An analysis of divergence based on isozyme patterns was performed by calculating genetic distances among the eight inbred lines. Based on the presence or absence of bands, the genetic distance was computed by using the Nie and Li coefficients. The dendrogram was obtained following unweighted pair group (UPGMA) method using arithmetic mean. Inbreds were classified into five groups A, B, C, D, and E. Analysis revealed that isozyme patterns in the inbred CM 600 was least similar to the isozyme patterns obtained in other inbreds. Similarity coefficients between CM 600 and CML 186 was least (0.4615) whereas, the highest (0.8511) existed between CML 142 and CML 144. Similarity coefficients ranged from 0.46 to 0.85, indicating moderate levels of diversity present among the inbreds under evaluation in the present study. Besides, this electrophoresis technique was fast, precise and without environmental effects. Thus, isozyme pattern based diverse group might be used in hybridization programme for obtaining heterotic hybrids.

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