Author(s):

Sudip Das, Diganggana Talukdar, Manjeet Kaur Sangha, Dharam Paul Chaudhary, Nilakshi Borah, Anamika Das, Subhendu Das and Siddhartha Proteem Saikia

Abstract: Activity of antioxidant enzymes viz. superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), glutathione reductase (GR) and peroxidase (POD) was estimated in leaves of barley and oat crops at 30, 60 and 90 days after sowing (DAS) under normal field condition. The antioxidant enzymes scavenge the reactive oxygen species generated during stress conditions. In barley, the APX activity (mmoles of MDA formed/min/g Fresh Weight of tissue) varied from 1742.53 ±92.00 at 30 DAS to 2160.6±42.25 at 90 DAS with highest value of 2917±49.94 at 60 DAS. Whereas the corresponding values recorded in oats were 473.94±615.28, 1923.72±13.16 and 1343.59±18.97 at 30, 60 and 90 DAS. The mean APX activity of all the three stages of barley was 1.84 times higher than the mean values of oat leaves. POD and CAT in barley leaves recorded mean values (three stages) of 116.16 E/min/g FW and 233.86 mmoles of H2O2 decomposed/min/g FW. These values were 1.74 and 1.676 fold higher than the corresponding values in oat leaves. Thus the present results are indicative of the fact that barley, a hardy crop is equipped with higher antioxidant enzymes and are thus better adapted to cope with drought

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