Author(s):

Rajendra Singh, KD Verma, Joginder Singh, Rashmi Nigam, Mahendra Singh and Anant Kumar

Abstract: The present studies according to findings, several naturally available substrates i.e. solid and liquid media were tested for mass multiplication of different Beauveria bassiana entomopathogenic fungus. The effect of different substrates for the mass production of B. bassiana spore/ml was significantly higher recorded were significantly producing spore per ml Data recorded on 7 day after the day after inoculation of various treatments in the T₁₀ Savoured dextrose broth (SDB), was the best treatment by bringing down the B. Bassiana production up to (48.3) spore/ml in liquid medium. Overall finding showed that different days among the liquid and solid media tested, for B. bassiana spore/ml production was significantly higher recorded 224.59 and 193.06 spore/ml were recorded on savoured dextrose broth (SDB) and potato dextrose broth (PDB). evaluated most appropriate medium for the production of B. bassiana potatoes, wheat flour, rice flour, corn flour and sugar cane molasses and solid phases includes sugar cane, corn, barley, rice, millet and sorghum. Among different media, sugar cane molasses extract and rice showed maximum growth of B. bassiana Different solid substrates i.e maize, bran, cotton seed, rice husk, wheat and liquid media such as coconut water were content and yeast extract concentration for mass production of entomopathogenic fungi: substrate for spore production. Collect the substrate for cost effective of B. bassiana spore/ml (1x10⁶) cost incurred for the production of spores T₁₀ Savoured dextrose broth (SDB) was the best low cost substrate and high production (Rs 5.43 and 149.35 spore/ml), T₁₁ Potato dextrose broth (PDB) (Rs 6.96 and 127.47 spore/ml), In the mass production of B. bassiana on different substrate solid and liquid medium “Savoured dextrose broth (SDB), Potato dextrose broth (PDB) and solid substrate _“Vermi compost + 1% YE + 1.0 g Dextrose” the best low cost substrate and high production found to be the best substrate in maximum spore production in minimum time as well as in cost of production of spore.

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