Objective: To quantify total phenolic, flavonoid contents in Phlogacanthus thyrsiflorus to evaluate the antioxidant potential along with HPTLC fingerprinting for identification of phytocompounds present in this medicinal plant.

Methods: Phytochemical screening, content of total phenolics and flavonoids were determined. The HPTLC fingerprinting of methanolic flower extract (MFE) of the Phlogacanthus thyrsiflorus was also carried out using CAMAG-HPTLC system.

Results: Phytochemical investigations revealed the presence of alkaloids, terpenes, tannins, saponins, glycosides and flavonoids. Total phenolic and flavonoid contents were found to be 65 µg/mg and 46 µg/mg, respectively. HPTLC analysis of MFE revealed numerous bands, indicating the presence of diverse groups of phytocompounds and also confirmed naringenin and ascorbic acid in the MFE sample.

Conclusions: It can be concluded that HPTLC fingerprint analysis of MFE of Phlogacanthus thyrsiflorus can be used as a diagnostic tool for the correct identification of the plant and it is also useful as a phytochemical marker.