Siddhapura Sanjay, Kunal N Odedra and BA Jadeja

The Physiological and biochemical seed vigour changes such as brick gravel test; Tetrazolium staining test, changes in protein content and reducing sugar are occurred in seeds during storage. Literature reported that these changes mainly depend upon the type of storage containers, variety of the seeds used and storage conditions. In the present study three different bags Cotton bag (C1), Jute bag (C2) and Polythene bag (C3) of were used for the storage of greengram seed of three different varieties Gujarat 4 (V1), GAM 5 (V2) and Virat (V3) under ambient temperature and relative humidity for a period of 18 months. The seeds from each container were taken after 3 months (90 days) and examined for different physiological and biochemical observations. 
Seed stored in polythene bag (C3) showed significantly higher brick gravel test germination (59.32%) as compared to those stored in jute bag (C2) (57.39 %) and cotton bag (C1) (54.28%) up to 540 days (T7) of the storage. Seed stored in polythene bag (C3) showed significantly higher value of Tetrazolium staining percentages (74.12%) as compared to those stored in cotton bag (C1) (68.87%) and jute bag (C2) (67.86%) up to 540 days (T7) of the storage. Maximum value for Tetrazolium staining percentages (74.12%) was observed in Gujarat-4 variety, followed by GAM-5 and Virat as 71.19% and 62.52% respectively at the end of 540 days of storage. The seed protein content was decreased in all three varieties Gujarat-4, GAM-5 and Virat i.e., 20.29%, 19.77% and 20.18% respectively after 540 days of storage. Seeds stored in polyethylene bag recorded maximum protein content as compared to cotton and jute bag. The reducing sugar content was decreased significantly in Gujarat-4, GAM-5 and Virat (0.97 %, 0.89% and 1.01%, respectively) after 540 days of storage. Seeds stored in polyethylene bag recorded maximum reducing sugar compared to cotton and jute bag at the end of storage. Thus, Gujarat 4 variety showed least effect on brick gravel germination and Tetrazolium staining and protein content during storage as compared to GAM 5 and Virat.
 

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