Vijaya Nirmala Pangi, Abhinash Marukurti, Alavala Matta Reddy, Rohini Anupoju, Phanindra Babu Kasi, Silpa Rani Medapalli, Inagadapa JN Padmavathi, Buddha Sai Keerthana and Shaik Farhana

Background: Plant produces tremendous diversity of secondary metabolites or chemical constituents which are of great importance to researchers in pharmacology field. The objective of this study was to evaluate the anti-vibriocidal activity and to determine chemical composition of methanolic leaf extract of Mirabilis jalapa L., the plant belongs to the family Nyctaginaceae.

Results: The preliminary qualitative investigation of M.jalapa leaf methanolic extract revealed the presence of Steroids, Coumarins, Alkaloids, Tannins, Glycosides and Phenols. The antibacterial activity was determined against Vibrio parahaemolyticus with zone of inhibition of 18.66 mm at 100 µg/mL concentration and Minimum Inhibitory Concentration (MIC) was found to be 62.5mg/mL. The chemical compounds or plant secondary metabolites ascertained based on molecular weights (m/z) obtained from gas chromatography-mass spectrometry chromatograms. The obtained spectrum peaks of GC-MS analysis was compared with stored database of National Institute Standard and Technique (NIST) library. A total of 29 chemical compounds were identified in GC-MS analysis, among these the highest peak area percentage was shown by Mome Inositol (46.89%) followed by Decanoic acid (16.64%) and its derivatives. Apart from these, two Phthalic Acid Esters (PAE’s) were also found in this study which is believed to be synthesized by plants, fungi, algae and bacteria according to the recent studies.

Conclusion: Evidently, some of these chemical compounds reported several biological activities which support the ethanomedicinal use of M.jalapa in treatment of various human ailments and diseases. The identified components in this study support the presence of phytopharmaceutical and ethanomedicinal versality of M.jalapa that could be used in the formulation of antimicrobial drugs.

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