Patel Naynika, Parikh Nirali and Mohan JSS

This work aims to evaluate the antimicrobial activity of n-Hexane, ethyl acetate, methanol and distilled water crude extract of selected medicinal plants. The potential of this extracts was analysed by agar well diffusion and two-fold serial dilution method against selected six gram-positive, six gram-negative bacteria and five fungi using positive control. Ethyl acetate flower extract of Raphanus sativus L. showed better antibacterial activity against EN (25mm), ST (24 mm), KP, SM and BC (22 mm). Good antifungal activity was observed in methanol extract of Raphanus sativus L. flower against AF(20mm), Cestrum nocturnum L. leaf against CA (20mm) whereas ethyl acetate extract of Amaranthus hybridus L. Var. paniculatus leaf inhibited AF (17mm), Ficus krishnae leaf CA(15mm) and D/W Indigofera sps whole plant extract inhibited AF (17mm). MIC and MBC value was observed in the range of 0.125mg/ml to >2mg/ml of selected crude plant extracts against tested organisms. HPTLC fingerprinting and bioautography of selected crude extracts demonstrated the presence of bioactive principle for antimicrobial activity.

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