Journal of Pharmacognosy and Phytochemistry
Vol. 10, Issue 2 (2021)
Morpho-cultural and pathogenic variability in Macrophomina phaseolina isolates from Soybean
Author(s):
Pankaj Kumar Mishra, Mala Kumari and Ravi Kant Dantre
Abstract:
Fifteen isolates of
M. phaseolina were characterized for cultural and morphological characteristics on PDA medium. Two fast growing isolates, seven medium growing isolates and six very slow growing isolates. The hyphal width of isolates was categorized in three groups. First group comprised of eight isolates with hyphal width ranging between 5-6 μm, second group 6-7 μm and third group more than 7μm. Mycelial growth pattern observed in isolates were fluffy, partial submerged, submerged, and irregular, whereas the colony colour found to be dark black, greyish and light greyish in appearance. Pathogenic variability revealed that, all the isolates were found to be pathogenic and diverse. Aggressiveness study through
in vitro blotter paper technique and cut stem inoculation technique exposed that variation exists within the isolates.
Pages: 935-943 | 979 Views 512 Downloads
Pankaj Kumar Mishra, Mala Kumari and Ravi Kant Dantre. Morpho-cultural and pathogenic variability in Macrophomina phaseolina isolates from Soybean. J Pharmacogn Phytochem 2021;10(2):935-943.