Author(s):

Sushmarani YS, P Venkatesha Murthy and Deeksha Raj N

Abstract: An efficient protocol for in vitro regeneration of Chrysanthemum (Dendranthema grandiflora T.) cv. Marigold was developed. In the present study shoot, tips and nodal segments were used as explants. Nodal explants responded better when compared to shoot tips. Explants were cultured on Murashige and Skoog (MS) medium with different concentrations of 6-benzylaminopurine (BAP) with Indole acetic acid (IAA). MS medium supplemented with BAP 1.0 mg L^-1 + IAA 0.1 mg L^-1 gave early shoot proliferation at 11.00 days after culture, maximum number of shoots per explant of 4.40, 8.01 and 10.36 and maximum shoot length of 3.43, 5.51. and 6.24 cm were recorded at 30, 60 and 90 days after shoot initiation. Satisfactory rooting response was obtained in half strength MS medium supplemented with 0.2 mg L^-1 indole butyric acid (IBA) took as early as 5.20 days after culture, and produced maximum number of roots per. Shoots of 4.49, 6.10 and 8.21 and root length of 3.85, 5.54 and 6.42 cm at 15, 30 and 45 days after root initiation.

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