Author(s):

S Kamalesh, J Suresh, L Pugalendhi, KB Sujatha and K Rajamani

Abstract: Java Tea (Orthosiphon stamineus Benth.) is an important medicinal plant most commonly used for its diuretic, hepatoprotective and antibacterial properties. Since, it is an emerging crop for large scale commercial cultivation, standardization of propagation using various types of cuttings and different concentrations of plant growth regulators were attempted in this study. Three types of stem cuttings viz., Terminal, middle and basal cuttings (15 cm length with 3-4 nodes) were prepared and treated with different concentrations of plant growth regulators viz., IAA (1000, 1500 and 2000 ppm) and IBA (1000, 1500 and 2000 ppm) for 3-5 seconds and subjected to rooting under mist chamber conditions. Observations on days taken for rooting, rooting percentage, number of roots, root length, fresh root weight, dry root weight, shoot length, number of shoots, fresh shoot weight, dry shoot weight and survival percentage were recorded. The results of the experiment portrayed that the basal cuttings treated with IBA 2000 ppm under controlled conditions resulted in better rooting percentage (95%), root length (11.68cm), fresh weight of root (0.94g), dry weight of root (0.32g), fresh weight of shoot (3.00g), dry weight of shoot (1.12g), number of shoots (2.53) and survival percentage (88%). Thus, this present investigation revealed that, the basal cuttings of java tea treated with IBA 2000 ppm under mist chamber conditions had significant effect on most of the root and shoot parameters and it was found to be most suitable for successful propagation of java tea.

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