Author(s):

Rajappa Vithal, Mina D Koche, Aparna Tekade and BT Raut

Abstract: Experiment was conducted in vitro to study the ability of different isolates to utilize carbon and nitrogen as essential element from different carbon and nitrogen sources, as its utilization depends on enzyme system. Infected chilli samples were collected from different regions of Vidarbha and Marathwada (Maharashtra), Bidar district (Karnataka) and Guntur district (Andra Pradesh) for isolation of Colletrotrichum capsici. Ten isolates of C. capsici isolated from collected samples. Eight different solid media viz., Asthana and Hawker’s, Richard’s, Czapek’s dox agar, oat meal agar, carrot agar, yeast extract-dextrose agar, nutrient agar and potato dextrose agar medium consisting various nutrients were tested and the observations were recorded on 3^rd and 7^th day of incubation. Statistical significance was obtained among the media in supporting the growth of isolates of C. capsici. The results revealed that significant differences among the different monosaccharides and disaccharides utilized by the isolates of C. capsici. All the sources of nitrogen are not equally efficient for supporting the growth of the fungi as they are specific in the utilization of ‘N’ sources. Among the different nitrogen sources, urea was found to support the maximum growth of eight isolates of C. capsici.

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