Author(s):

Basant, Parma Ram Gorachiya, Rekha Panwar, BN Shringi and Amit Kumar Pandey

Abstract: The present study aimed to designing of synthetic peptides as efflux pump inhibitors and determination of their efficacy along with some antibiotics. Two synthetic peptides SG-15 and GG-15 were designed on the basis of protein sequence and structure of AcrAB-TolC a commonly expressed efflux pump of Escherichia coli (E. coli). Efficacy of both the peptides were evaluated by determination of their synergistic effect with three antibiotics namely; Ampicillin, Gentamicin and Cephalothin through minimum inhibitory concentration of on 10 E. coli isolates obtained from mastitic milk samples of cattle. Designed synthetic peptides of present study were proven potent efflux pump inhibitors as SG-15 was able to decrease in MIC of an antibiotics upto 8-16 folds. Discovery of such agents are becoming hope in the treatment of newly emerging multidrug resistant strains of several common pathogenic bacteria.

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