It includes the collection of isolates from AICRP on mushroom, college of Agriculture Pune. After collection its cultural variability on different media was studied different pH levels and different temperature regimes were studied. Three culture media (PDA, MEA and Richard’s Agar) were tested for growth of different Pleurotus spp. Among them growth of PN-18-10 was significantly more on Malt extract agar (70.40 mm). Whereas, growth of PN-17-31 were significantly more on potato dextrose agar (44.12 mm) and growth of PN-17-23 significantly more on Richard’s agar media (50.44 mm). All eleven isolates grown on 5,6,7,8, and 9 pH levels and among them pH 6 showed optimum growth rate for All eleven isolates. OnpH 6.0 growth was observed about 34.11 mm followed by pH 8.0. On pH 5.0 PN-18-of oyster mushroom were tested for effect of different range of temperature viz., 20 ⁰C, 25 ⁰C, 30 ⁰C, 35 ⁰C, 40 ⁰C and found that they were grown well at 30 ⁰C temperature with maximum mycelial diameter 56.00 mm in P. cystidiosus, 87.00 mm in P. populinus and 48.00 mm in P. ostreatus.

Among the cultural variability variation due to different media different pH levels and different temperature regimes were studied. Among the tested eleven isolates of oyster mushroom all isolates showed significant growth on MEA media followed by PDA media. For the optimum pH level, pH6.0 found optimum for the growth of mycelia of most of the Pleurotus isolates.