Author(s):

Raman Lukashou, Natalia Gurina, Ludmila Lyubakovskaya and Аngela Grigorovich

Abstract: Five hydroxycinnamonic acids were found and the presence of chlorogenic and caffeic acids was confirmed. The dominating compound is caffeic acid. The following optimal conditions of water-ethanol extraction of the total hydroxycinnamonic acids from brown knapweed are experimentally selected: volume ethanol fraction – 80%; extraction time – 6 hours; extraction temperature – 80ºС; extraction multiplicity- three times; the ratio of brown knapweed and 80% ethanol – 1:50; the degree of brown knapweed grinding – 500 μM (microns). The nature of the extraction of the total hydroxycinnamonic acids is determined by the dominating compound – caffeic acid. Total content of hydroxycinnamonic acids in brown knapweed taking into account the selected optimal extraction conditions makes, on the average, 2.6%. Volume ethanol fraction; time, temperature and multiplicity of extraction statistically significantly affect the concentration of hydroxycinnamonic acids in the extractions obtained. The most critical factor of water-ethanol extraction is extraction time.

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