Author(s):

Tapash Kumar Bhowmik and Md Mahbubur Rahman

Abstract: Spathoglottis plicata Blume is an ornamentally and medicinally significant terrestrial orchid of Bangladesh. This orchid seeds can be asymbiotically germinated by in vitro process for fast propagation. Varied response was found in terms of seed germination, protocorm like bodies formation and seedling development was observed on four different basal media. Medium supplemented with PGRs favored optimum condition for the germination of seeds followed by full strength and half strength on KC, MS, PM and VW media. Based upon the results, it was found that MS medium was more effective than KC, PM and VW media for germination, PLBs and plantlet formation. PM medium with PGRs combination gave the maximum percentage response (93.34%) followed by PGRs supplemented MS (80.00%), VW (73.34%) and KC (73.34%) media. Minimum percentage of seed germination was observed on half strength PGRs free VW (26.67%) medium. Minimum time needed for initiation of germination in full strength PGRs supplemented PM (8.67 ± 0.36a weeks) medium, whereas, maximum time required (20.17 ± 0.38h weeks) in PGRs free half strength KC medium.

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