Author(s):

Manitriniaina Rajemiarimiraho, Louise Faramalala Rabemandroso, Lahadson Stanislas Fulgence, Volasoa Herilalaina Victorine Rambeloson, Anne Wadouachi, Amélie Raharisololalao and Léa Herilala Rasoanaivo

Abstract: The latex of Anthostema madagascariensis is used as a purgative in Malagasy traditional medicine. The study was aimed to investigate the laxative activity of stem barks extracts of A. madagascariensis. The hydroalcoholic crude extract of the stem barks from A. madagascariensis and the three fractions resulting from the fractionation of the hydroalcoholic extract and the isolated compound from the active extract were tested. The laxative activity was investigated using in vivo models intestinal motility in mice. Among all tested dose, the hydroalcoholic extract more or equal to 1000 mg/kg and ethyl acetate extract at 1200mg/kg produced significant (p<0.05) increase in the intestinal motility in the treated mice when compared to the negative control. The fractionation of the ethyl acetate extract resulted in the isolation of triterpene namely epi-betulinic acid. Its structure was established by spectroscopic methods. This compound at dose 10 mg/kg produced significant (p<0.05) increase in the intestinal motility in the treated mice when compared to the negative control. It is for the first time that laxative activity of epi-betulinic acid was demonstrated.

Pages: 2086-2090  |  676 Views  261 Downloads

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