Author(s):

Dr. Bhuwan Chandra Sharma, Dr. Rajesh Pratap Singh, Dr. Renu Singh and Dr. Pradeep Kumar

Abstract: Maize (Zea mays L.) is one of the most important cereal crop in the world. It is grown throughout the temperate, tropical and sub tropical zones and has worldwide significance as human food, feed for live stock and for industrial and pharmaceutical sectors as every part of maize plant has one or the other economic values. Banded leaf and sheath blight (BL&SB) caused by Rhizoctonia solani f. sp. sasakii kuhn Kühn is one of the important disease of maize that became serious in recent years and recognized as a major constraint for limiting production. This pathogen causes losses in grain yield to the extent of over 90 percent. Pantnagar has been recognized as a hot spot for banded leaf and sheath blight disease at national level. Present study was undertaken to see the in vitro effect of fungicides and bioagents against the pathogen. In vitro, Efficacy of fungicides against test pathogen was studied by poisoned food technique. The fungicide viz, propiconazole and carbendazim were evaluated at 5 different concentrations viz 1, 5, 10, 20 and 50 µg/ml where as mancozeb at 20, 50, 100, 200 and 300 µg/ml. The Efficacy of three bioagents viz Trichoderma harzianum, Pseudomonas fluorescens and Trichoderma harzianum + Pseudomonas fluorescens was tested using dual culture technique against test pathogen. Fungicides Propiconazole and Carbendazim @ 20 µg/ml completely inhibited the radial growth of test fungus where as in case of Mancozeb it was achieved @ 100 µg/ml. Among the bioagents maximum percent inhibition in mycelial growth of test pathogen was recorded in Trichoderma harzianum + Pseudomonas fluorescens (62.35%), followed by Trichoderma harzianum (44.41%) while least percent inhibition was recorded in Pseudomonas fluorescens (41.18%).

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