Author(s):

S Karpagavalli and Nannapaneni Kiran Kumar

Abstract: Trichoderma viride is a potential biocontrol agent for plant disease management especially seed borne and soil borne diseases. Trichoderma is a free living saprophytic fungus with high Competitive Saprophytic Ability (CAS), soil inhabitant and also survived on aerial parts of the plant. Biological control alone will not be feasible to combat the disease in case of severe incidence. Based on high cost and environmental concerns it is not advisable to protect the crops for the entire period by conventional fungicides. Biological control, in integration with fungicidal treatment was more reliable approach to manage soil borne and seed borne pathogens. In the present study the native isolate of T.viride was used for assessing their growth on fungicide amended medium (poisoned food technique). The fungicides viz., carbendazim (0.1, 0.2 and 0.3 per cent), copper oxy chloride (0.2, 0.3 and 0.4 per cent), thiophanate methyl (0.05, 0.1 and 0.2 per cent), benomyl (0.05, 0.1 and 0.2 per cent) and sodium hypochlorite (5, 10 and 15 per cent) were used. Mycelial growth in fungicide amended solid medium and mycelial dry weight in fungicide amended liquid medium was studied to assess the compatibility. The radial mycelial growth of T.viride was significantly reduced by the fungicides at all concentrations tested. Among them, copper oxy chloride showed minimum inhibition of 45.55 per cent in mycelial growth and maximum weight of 77.00 mg of mycelial dry weight at 0.2 per cent concentration.

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