Author(s):

Saifur Rehman Khattak, Amjad Hussain, Aadil Ameer Ali, Taufiq Ahmad, Sultan Ayaz, Muhammad Akram, Muhammad Ishaque MR and Wahid-ullah

Abstract: Moringa oleifera has been used extensively for its nutritious and therapeutic value. Numerous parts of the plant such as flower, root, leaves, seeds and bark are used for these purposes. In Pakistan the use of moringa plants is not very common, however, due to some scientific work and subsequent promotion by some workers its use is gaining importance day by day. The current study has been undertaken to scientifically explore some aspects of the plant particularly leaves and bark by using chromatographic techniques such as TLC and HPLC. The Thin layer chromatography (TLC) separates only two vitamins (vitamins B2 and B6) with the help of methanol and Ammonia solution (100:1.5 v/v) as a standard mobile phase whereas the HPLC have cause separation of vitamin B1 (Thiamin), B2(Riboflavin), B3(Niacin), B6(pyridoxine) and Ascorbic acid (vitamin C).

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