Author(s):

RK Ranjan and Dinesh Singh

Abstract: Bacterial wilt (Ralstonia solanacearum) infected potato samples collected from Odisha (cv. Kufri Pukhraj) and Uttarakhand (cv. Kufri Jalandhar), to study decay of potato tubers, other pathogens associated with potato tubers and survivability of R. solanacearum during storage at different temperatures (5, 15, 25 and 35 ^oC) up to 60 days. Minimum decay of stored potato of Kufri Jalandhar (5.82%) and Kufri Pukhraj (7.53%) was found at 5^oC followed by 15^oC after 60 days of storage. R. solanacearum, Erwinia spp. and Bacillus spp. were found to be common in rotted potato of both the potato cultivars Kufri Pukhraj and Kufri Jalandhar. In case of fungi, Fusarium oxysporum, Mucor genevensis and Penicillium sp. were common in both potato cultivars while Macrophomina phaseolina was recorded only on Kufri Pukhraj cultivar. Study on survivability of R. solanacearum in stored potato tubers cvs. Kufri Pukhraj and Kufri Jalandhar at 5, 15, 25 and 35^oC temperatures up to 60 days by dilution plate technique, conventional-PCR and RT- PCR methods. The population of R. solanacearum was found maximum at 5 ^oC (6.27 cfu log/g tissue and 6.20 cfu log/g tissue) followed by 15 ^oC and minimum at 25 and 35 ^oC stored potato samples in both the potato cultivars Kufri Pukhraj and Kufri Jalandhar by serial dilution plating techniques respectively. Conventional PCR results also confirmed the detection of R. solanacearum at 5 and 15^oC stored potato which was gradually increased up to 60 days of storage in both the cultivars, whereas the samples stored at 25 and 35^oC the detection of R. solanacearum was gradually declined after 20 days of storage. The detection and quantification R. solanacearum population by RT –PCR, maximum population of R. solanacearum was recorded in 5^oC stored potato followed by 15 ^oC. The C_T value of R. solanacearum was 26.90 at 5^oC and 34.67 at 35^oC in Kufri Pukhraj whereas 25.70 at 5 ^oC and 31.70 at 35^oC in Kufri Jalandhar after 60 days of storage.

DOI: 10.22271/phyto.2020.v9.i3r.11439

Pages: 1103-1109  |  710 Views  260 Downloads

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