Methods: Different types of plants are used for containing aqueous extract of Neem (Azadirachta indica), Clove (Syzygium aromaticum), Betel (Piper betle), Turmeric (Curcuma longa), Peppermint (Mentha piperita), and Guava (Psidium guajava) was used for phytochemical screening and antibacterial activity against S.aureus, B. subtilus and E. coli bacteria and identification test of inhibition zone using with different concentration of extract were used (100 mg/ml).

Results: Antibacterial activity of Neem (Azadirachta indica), Clove (Syzygium aromaticum), Betel (Piper betle), Turmeric (Curcuma longa), Peppermint (Mentha piperita), and Guava (Psidium guajava) are used as an aqueous extraction of Crude drugs against S.aureus, E.coli and Subtilus.

Conclusion: These result shows the medicinal plant extract is promising phytochemical analysis and antimicrobial effects against both gram positive and gram negative bacteria. We are used all medicinal plants are very useful for human in the present time. All plants are very good source of Antimicrobial, Antifungal, Analgesic, Antiseptic etc.