Author(s):

Rathore Indu, Kaushik RA, Rajpurohit Deepak, Maloo SR and Chittora Manish

Abstract: Chrysanthemum (Compositae) is the second largest cut-flower after rose among the ornamental plants traded in the global flower market. It is propagated vegetatively as it has a strong sporophytic self-incompatibility system as shown by all members of Asteraceae family. Chrysanthemum grandiflora is a hybrid species which is the result of repeated cycles of inter specific crossing among elemental species bear blooms with two types of florets arranged on a flattened axis called capitulium. The outer florets are called ray floret and inner florets as disc floret. The ray florets have only female parts while disc floret may have both male and female parts and a tubular made up of five united petals. Being a cross pollinated crop, a lot of variation is present in chrysanthemum varieties. Twelve random amplified polymorphic DNAs (RAPDs) and eighteen and inter simple sequence repeats (ISSRs) markers were used for the evaluation of genetic variation and to assess polymorphism of cultivated chrysanthemum tested the genetic stability of chrysanthemum. The results indicate that the rate of polymorphism showed significant differences as compared to other existing varieties. The average number of total polymorphic bands were 3.78 for RAPD and 2.94 for ISSR markers. The size of RAPD and ISSR amplified fragments varied from 100bp – 2000 bp. Therefore RAPD allow the rapid detection of DNA polymorphisms, from many individuals or pooled samples. The advantages of RAPD lies in its simplicity, rapidity, requirement for only a small quantity of DNA and the ability to generate numerous polymorphisms. Thus it is considered a powerful technique for genetic analysis ISSR technique independently or in combination with other markers is used for genetic characterization of different plant species as an alternative reliable technique to study genetic diversity based on the presence of micro-satellites throughout genome.

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