Author(s):

ST Pawale, VP Chimote and MP Deshmukh

Abstract: Soybean [Glycine max (L) Merrill,] contains 38-44% Protein and 18-23% oil, which makes it unique among the cultivated crops. Presence of KTI is governed by a single dominant gene (Ti), while the absence is attributed to the recessive form (titi), which encodes for truncated protein. In soybean, there are ten independent differentially expressed KTI genes; of which KTI₃ is seed specific (Moraes et al., 2006) ^[8]. The recessive null kti allele contains one substitution and two deletions, which alters the translation process resulting in reduced levels of KTI in seed embryos (Jofuku et al., 1989) ^[5]. Kim et al., (2006) had reported that three SSR markers viz., Satt 228, Satt 409 and Satt 429 to be closely linked (0-10 cm) with the Kunitz trypsin inhibitor gene. After PCR amplification, the PCR products were resolved on 1% agarose gel electrophoresis for gene specific primer and 3% agarose gel electrophoresis for Satt 409 primer. Charcoal rot caused by (Macrophomina phaseolina) is the second largest yield reducing disease after brown spot (Septoria glycines) in the soybean growing countries across the globe. The fungus causes a general root rot in soybean, infecting the roots and lower stems. SSR Marker: Satt 640, Satt 542 and Satt 301 has been reported resistance to charcoal rot in segregating population. This disease reported under polygenic control. Heritability is also reported to be low. Charcoal rot is a disease that appears in hot and dry weather when soil temperatures are 80-90 °F (27-35 °F) for 2 to 3 weeks. The disease has been an endemic problem in relatively dry or drought-like situations.

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