Author(s):

Rakhi Kumari, Dr. AK Pal and Sunil Singh

Abstract: Seeds of cowpea variety Kashi Kanchan were treated with gamma rays, EMS and combination treatment of gamma rays with EMS to obtain the spectrum and frequency of chlorophyll mutations in M2 generation in the present investigation. The individual treatment of Gamma rays was found to be more efficient than EMS to induce chlorophyll mutants. A progressive increase in mutation frequency of chlorophyll mutations was observed with increasing doses. Four different types of chlorophyll mutants, such as, albino, xantha, chlorina and viridis were induced with effect of mutagens. The highest frequency of chlorophyll mutations (2.87) was reported in the Gamma rays. There was a dose dependent increase in the spectrum and frequency of chlorophyll mutations whether mutagens were employed singly or in combination up to a certain dose / concentration level beyond which it decreased at higher frequency.

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