Author(s):

Manorama Kumari, Anusha Kokkiligadda, Pradip V Behare and SK Tomar

Abstract: We investigated environmental stresses tolerance of the extracellular vitamin B₁₂ producing lactobacilli cultures and confirmed the screening strategy of vitamin B₁₂ producing Lactobacillus strains, isolated from the infants’ fecal sample. Three cultures (L. plantarum NCDC958 and NCDC957, and L. rhamnosus NCDC953) endowed with extracellular vitamin B₁₂ producing ability from our previous work were amplified for cbiK gene with new primers designed in this study and their characteristics in tolerance to environmental stresses (such as temperature, salt, sugar, and ethanol) were studied for the first time with both optical density (OD) measurements and pour plate count method. We confirmed amplification of the 257 bp cbiK gene in these cultures, which codes for important B₁₂-structural enzyme cobalt chelatase and successfully selected B₁₂-producing strains. These cultures showed tolerance upto 6% NaCl, 30% sucrose, 42 °C temperature and 8% ethanol. Importantly, extracellular vitamin B₁₂-producing culture L. rhamnosus has first-time been reported for the environmental stress tolerance.

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