Author(s):

Priyanka Tirkey and Devi singh

Abstract: A field experiment was conducted at Department of Horticulture, Sam Higginbottom University of Agriculture, Technology & Science, Prayagraj, India during the year 2016-17.The experiment was laid out in randomized block design on traits of gladiolus traits of gladiolus ‘White Prosperity’, bulbs were induced by gamma rays, Dry Ethyl Methane Sulphonate (DEMS) and soaked Ethyl Methane Sulphonate (SEMS). The treatments consisted of 2 Kr, 4 Kr, 6 Kr, 8 Kr, 10 Kr and 12 Kr of gamma rays; 0.25%, @ 0.5%, @ 0.75%, @ 1.0% and @ 1.25% of DES; 0.25%, @ 0.5%, @ 0.75%, @ 1.0% and @ 1.25% of EMS and control (untreated).. Different concentrations of EMS and different level of gamma radiations had a little effect on sprouting and survival percentage but it significantly delayed the emergence of sprouts. About one third decreases in plant height along with reduction in number of leaves per plant, leaf area, length and width, diameter and number of cormles per corm was observed in treated plants. In general, the treated population had manifested reduced expression than the control (untreated population) for most of the characters. Higher the dose of mutagens, lower was the expressivity of the traits. After the observation it found that maximum plants height was observed in control while minimum height observed in the treatment with Gamma Rays 12Kr while maximum number of leaves and number of shoot per corm were recorded in treatments with Gamma Rays 2Kr and SOAKED EMS @ 0.25%) respectively. The gladiolus plants could not survive in treatments of gamma rays and its combination with EMS. On the basis of above observations, it may be concluded that 0.25 to 0.0.50 per cent dry and soaked EMS doses are suitable for inducing mutation in gladiolus.

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