Author(s):

P Suresh, K Poornima, S Nakkeeran, P Kalaiarasan and RM Vijayakumar

Abstract: Guava (Psidium guajava L.) is an important fruit crop cultivated throughout India. Cultivation of guava is affected due to the outbreak of nematode fungal disease complex. Fusarium solani is a widely distributed soil inhibiting fungus that causes wilt disease in guava. In the present investigation, six isolates of wilt pathogen, Fusarium species were isolated from wilt infected guava roots from Karamadai, Coimbatore district. Pathogenicity studies conducted on guava seedlings (Var L- 49) revealed that, two out of the six isolates of F. solani were found to be pathogenic. Molecular characterization of two isolates (F1 and F3) were carried out by amplification of Internal Transcribed Spacer (ITS) region of the conserved ribosomal DNA using primers ITS1 and ITS4. The amplified sequences were submitted in NCBI (F1- MH590324 and F3-MH590695) and phylogenetic relationship of closely related organism of Fusarium spp. were carried out.

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