Author(s):

Sangeetha Sathyanarayan and K Sadasivan Pillai

Abstract: Type 2 diabetes mellitus once considered as a metabolic disorder characterized by chronic elevation in both fasting and postprandial levels is now established as a polygenic disease involving several etiological factors. Alpha amylase and alpha glucosidase inhibitors are used to achieve greater control over hyperglycemia in type 2 diabetes mellitus. Polyherbal preparations with various active principles and properties have been used in different regions and cultures to treat a wide range of human diseases. In the present study an attempt has been made to evaluate the antioxidant and in vitro antidiabetic efficacy of GOA 111, a mixture of Gymnema sylvestre, Ocimum sanctum and Azadirachta indica in the ratio of 1:1:1. Polyherbal formulation was analyzed for phytochemicals. The antioxidant potential of GOA 111 was determined using DPPH, ABTS assay. The effect of GOA 111 on the activities of alpha glucosidase and alpha amylase were studied. GOA 111 contains Alkaloids, flavonoids, tannins, saponins, glycosides, steroids, phenols, resins, Coumarins, proteins and carbohydrates. Its shows significant amounts of total phenol, flavonol and flavonoid. There was a significant percentage inhibition of 74% and 72% at a concentrations of 1000µg/ml for DPPH and ABTS assays respectively. GOA 111 showed 58 % inhibition and 72% inhibition for alpha amylase and glucosidase assays at a concentration of 500 µg/ml. The formulation, in different concentrations, is able to enhance uptake of glucose in yeast, the simplest eukaryotic cells. Hence it can be concluded that Goa 111 possess significant antidiabetic and antioxidant properties.

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