Author(s):

SN Upadhyay, DH Patel and HR Patel

Abstract: Knowledge of genetic diversity and relationship among breeding material at molecular level has significant impact on cotton improvement. In the present study genetic variability and relationships among 11 cotton genotypes was studied using RAPD markers. Twenty five RAPD primers generated a total of 123 alleles, of which 71 alleles were found to be polymorphic, resulting in 57.72 % polymorphism. The similarity coefficient based on RAPD markers ranged from 0.44 to 0.87 with an average of 0.71, thus suggesting considerable genetic variation among the cotton genotypes studied. Further, dendrogram generated by UPGMA cluster analysis based on Jaccard's similarity coefficient, grouped 11 parental lines into two distinct clusters. The grouping based on clustering analysis revealed that genetic relationship of several varieties is related to their centres of development. Our result suggests that combined molecular markers data are efficient for measuring genetic diversity, relatedness and also aid in selection of diverse outstanding lines to be used in future breeding programs to develop new cotton hybrids/cultivars.

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