Author(s):

Sandeep Kumar, SP Singh, Shivam Singh, Jay Kumar Yadav, Vikas Upadhyay, Dibya, Ankur Katiyar and Arun Kumar Jaiswal

Abstract: Spot blotch in wheat is mainly caused by Bipolaris sorokiniana (Sacc.) in India and neighboring South Asian countries and capable of causing losses in yield up to 50% in susceptible varieties as well as results poor grain quality. Bipolaris sorokiniana of wheat was investigated with relationship between morphological and pathological variability of isolates on different media namely PDA, Oat agar, Corn agar media and Standard blotter. Isolates were collected from the leaves of field-grown wheat crop at Main Experiment Station, NDUA and T, Kumarganj, Ayodhya. Morphological characters—i.e., colony morphology, growth, color and pathogenic characters – i.e., shape and size, number of septa and color. The single spore cultures were later inoculated on a different media such as PDA, Oat agar, Corn agar media and Standard blotter and incubated at 25±1ºC at 85-95% humidity inside B.O.D. for 7 days. Three types of colony were obtained from the infected wheat leaves. Characteristics of these pure cultures were studied after 7^th day of isolation. Colony obtained from wheat leaves were suppressed, dark grey to brownish black in colour with smooth to wavy margin and colony diameter varies from 6.75-9.00 cm. In the initial stage olive, dark brown like mycelial growth occurred on potato dextrose agar medium which later on changes into olive green or gray coloured. After seven days of growth, mycelium becomes dark gray and it was light gray at margins. In petri dishes, fungus develops into clear zones with alternation of dark olive and light brown colour. In 15 day old cultures, mycelium becomes dark greenish grey or black coloured at margins with abundant sporulation. Development of fresh white mycelium on the dark greenish grey mat of mycelium was also observed.

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