Author(s):

Sweta Mishra, Shailesh Kumar and Yuvraj Vala

Abstract: The present study determined the effect of combination of coconut water, cytokinin and light intensity for enhanced direct in vitro organogenesis of olives (Olea europaea L.). This optimized protocol showed that coconut water and light intensity are important factors for efficient shoot regeneration. These results show that the light intensity and the natural additive, coconut water olds the potential for efficient commercial in vitro propagation of olives. The meristematic explants were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of coconut water, 2ip and kinetin under light intensity of 20, 30 and 40 μmol m⁻² s⁻¹. The highest shoot induction (93.16 ± 0.09%) was obtained with 1.5 mg l⁻¹ Kinetin + 2.0 mg l⁻¹ 2ip under a light intensity of 30 μmol m⁻² s⁻¹. The shoot proliferation and shoot length was highest (08.73±0.18 and 09.52±0.14, respectively) on media with 1.0 mg l⁻¹ Kinetin + 2.0 mg l⁻¹ 2ip and 10.0 % coconut water under a light intensity of 30 μmol m⁻² s⁻¹. The highest root induction (92.28±0.12 %), rooting frequency (07.94±0.16 roots per shoot) and root length (07.73±0.17 cm) were obtained when adventitious shoots were inoculated on half-MS medium with 1.0 mg l⁻¹ 2,4-D + 1.0 mg l⁻¹ NAA. The regenerated plantlets were acclimatized in coco peat, soil and sand mix for 15 days and were shifted to poly house for 2.5 months and thereafter they were transferred to field. The survival rate of the transplanted plantlets was about 85%.

Pages: 846-850  |  1300 Views  558 Downloads

Download (546KB)