Author(s):

Chavan Narendra Rameshsing, Kale Sonam Sureshrao, Kadu Tanvi Pradiprao, Toshy Agrawal and SB Verulkar

Abstract: DNA markers have enormous potential to improve efficiency and precision of conventional plant breeding via MAS. QTLs mapping studies for diverse crop species have provided an abundance of DNA marker trait associations. Two F3 populations with parental designation of Dagaddeshi (Land race) was tolerant to drought and MTU1010, is the most popular high yielding variety in central India, however is susceptible to water stress (Drought), and used for validation of root and yield related markers in kharif-2015. These populations were evaluated under stress and non-stress conditions, SSR linked markers within DTY QTL 1.1 present on chromosome #1, chromosome # 5. A total 40 SSR linked markers within DTY QTL 1.1 present on chromosome #1, chromosome # 5 and some markers adjacent to RM 242 regions on chromosome # 9 were used for validation. Among 40 markers 2 markers were linked to chromosome 1, 3 markers for chromosome no #5, 5 chromosomes for QTL on chromosome 9 adjacent to RM 242. A total of 10 markers were used for validation studies. The marker, RM242 showed 1% significant association with seedling height during the irrigated (transplanted) condition whereas flag leaf width showed 5% significant association and TSD (transplanted) RM242 showed 5% significant association with total root length.

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