Objectives: The present study evaluated pharmacognostic and physicochemical standards.

Methods: Macroscopy, microscopy, phytochemical screening, Thin layer chromatography were carried out

Results: Macroscopy reveals the organoleptic properties of the fruit and microscopic study shows the presence of oil glands and vessels responsible for essential oil production in rind. Water soluble ash value is more than acid insoluble ash indicates that the acid-insoluble siliceous matter was less than that of water soluble ash. Thin layer chromatography of methanolic extract has been carried out and result was mentioned as R_f value. The preliminary phytochemical investigations indicate the presence of carbohydrates, amino acids, flavonoids, tannins and phenolic derivatives, steroids etc.

Conclusions: Pharmacognostic and phytochemical parameters of Citrus medica fruit can be considered as distinctive enough to identify the authenticity of fruit.