Author(s):

Abirami T, Bavani Govindarajulu and Karthikeyan J

Abstract: Multidrug resistant strains of microorganisms are the major threat to the treatment of infectious disease. Andrographis paniculata leaf extract and its mediated synthesized silver nanoparticles were evaluated against Bacillus cereus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa. Antibacterial activity was studied using disc diffusion method using sterile discs. The bactericidal efficacy was recorded as zone of inhibition measured after overnight inhibition with specific concentration of extracts. The green synthesized silver nanoparticles were characterized using UV-Spectroscopy and Scanning Electron Microscopy (SEM). The alcoholic extract shows moderate activity against the tested organisms when compared with aqueous extract however, a significant zone of inhibition of 12mm to 17mm was recorded with reference to 10µg/ml concentration of green synthesized silver nanoparticles. The zone of inhibition recorded was higher than the positive control amoxicillin. To conclude, Andrographis paniculata mediated synthesized silver nanoparticles shows better efficiency as bactericidal agent when compared with ethyl acetate and aqueous extract of the tested plant and the silver nanoparticles coated with bioactive compounds of the plant material can be function as a better alternative to control the drug resistant bacteria in place of synthetic medicines used to control or treat the bacterial infection.

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