Author(s):

Pravin Morankar and Alok Pal Jain

Abstract: Diabetes mellitus has become a major health concern worldwide and development and progression of diabetic related complications are accelerated by reactive oxygen species which are generated and accumulated due to hyperglycemia. Herbal remedies are convenient for the management of diabetes due to their traditional acceptability and availability, low costs and lesser side effects. The present study was carried out to determine the in vitro antioxidant activity, antidiabetic activity and HPLC analysis of methanol extract of Clematis heynei and Solanum virginianum. Antioxidant activities of methanolic extract of C. heynei and S. virginianum were evaluated by H₂O₂ and No scavenging assay and antidiabetic activity was determined by in-vitro alpha-amylase inhibition assay and quercetin was detected in methanolic extract of C. heynei and S. virginianum under study by using RP-HPLC analysis. C. heynei and S. virginianum extracts showed effective hydrogen peroxide radical scavenging and nitric oxide scavenging activity. The antioxidant activity was increased dose dependently when compared with standard drug ascorbic acid. In in-vitro antidiabetic test, highest α-amylase inhibition was found in methanolic extract of C. heynei then S. virginianum extract. Quercetin content was highest in C. heynei extract, was quantified as 0.820µg/ml and in S. virginianum extracts 0.798µg/ml. HPLC analysis of plant extracts indicated the presence of the most abundant dietary flavonol, Quercetin. The results of this study revealed that the bioactive compound content differences could be determinant for the medicinal properties of this plant especially for antioxidant and antidiabetic activities with potential applications in food and pharmaceutical industries.

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