Author(s):

Ajay Kumar Chandra, Kundan Kishor Rajak, Kavita Gururani, Harsh Kumar and Mithilesh Kumar

Abstract: The present study was conceptualized to study the effect of explants and plant growth regulators on organogenesis to establish an efficient regeneration protocol for Coleus barbatus, an endangered ethnomedicinal Plant. In course of this, sterilized explants were cultured onto MS medium augmented with different concentrations and combinations of auxin (IAA) and cytokinin (BAP). The best callogenic response was observed on media M₄ followed by M₉ and M₅ in terms of Days of callogenesis, degree of response, colour and texture, considering all the three explants. Regenerated shoots from embryogenic calli were further multiplied on fresh media. Highest per cent shoot regeneration response was obtained on medium M₁₂ (84.7%) and M₇ (84.7%) followed by media M₆ (81.4%), whereas in terms of number of shoots per culture, shoot apex (0.0-3.7) and internodal stem (0.0-3.7) exhibited as better explant then young leaf (0.0-1.7). The long and healthy shoots were harvested and subcultured on rooting media for root induction. Maximum per cent response of root proliferation were obtained on medium M₁₃ (85.2%) followed by media M₉ (84.4%) and M₈ (84.1%), whereas for highest frequency of root per elongated shoot, shoot apex (0.0-10.3) exhibited as better explant then internodal stem (0.0-9.7) and leaf explants (0.0-7.3). Hence, the In vitro regenerated plantlets using this established micropropagation protocol can be used for large-scale commercial cultivation, in situ conservation, and genetic characterization of this endangered medicinal plant.

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