Author(s):

Nagaraja KS, Praveen Jholgiker and Dr. GSK Swamy

Abstract: Multiple in vitro shoot cultures of two banana cultivars viz., Rajapuri (AAB) and Grand Naine (AAA) were treated with EMS (Ethyl Methane Sulphonate) at different concentration (Control, 0.1%, 0.2%, 0.3%, 0.4% and 0.5%) to create genetic variability through mutation. The interpretation of data pertaining to shoot multiplication ratio revealed that there was significant differences for EMS treatment at I, II and III sub culture stage. The maximum shoot multiplication ratio was observed in water treated plantlets which was found statistically on par with plantlets treated with 0.1, 0.2 and 0.3 per cent of EMS in all the stages of sub culture. However the lowest shoot multiplication ratio was observed in plantlets treated with higher concentration of 0.5 per cent EMS. There was no dwarfing mutants in the plantlets treated with 0.1, 0.2 and 0.3 per cent EMS concentration in all the subculture stages. However, the maximum per cent dwarfing mutants was found in plantlets treated with 0.5 and 0.4 per cent EMS concentration in all the culture cycle. Interactions at II subculture suggested that, Rajapuri plantlet treated with 0.5 per cent EMS concentration had maximum dwarfing mutants. The interpretation of data pertaining to per cent survival among plantlets exposed to different concentration of EMS revealed fewer variations. There was no mortality in plant culture treated with 0.1, 0.2 and 0.3 per cent EMS concentration and control (water treated plant culture). Where as there was marginal reduction in per cent survival of plantlets in plant culture treated with 0.4 and 0.5 per cent EMS.

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