Author(s):

Ved Ratan, Mukesh Srivastava, Supriya Dixit, Shubha Trivedi, DK Srivastava and Yatindra Kumar Srivastava

Abstract: Recently, the environmental contamination caused by excessive use of chemical pesticides increased the interests in integrated pest management, where chemical pesticides are substituted by bio-pesticides to control plant and soil diseases. Trichoderma species are well known potential fungal bio-control agents against a wide range of soil borne plant pathogens. A total 25 Trichoderma isolates were isolated from soil samples collected from Kanpur Nagar, Kanpur Dehat, Unnao and Lucknow districts of U.P. Based on cultural and morphological characters, six Trichoderma isolates found efficient and taken for further studies. All six isolates were sent to NFCCI & FIS, Pune for species level identification. Based on the identification report from NFCCI, 4 isolates were identified as T. asperellum (CST-02, CST-09, CST-21, CST-22), one each as T. longibrachiatum (CST-14) and T. koningii (CST-05). The in vitro antagonistic activity of the 06 Trichoderma isolates was assayed against both test pathogens. Our results showed that the highest inhibitory effect was achieved by isolate CST-14 on growth of FOC and FOL as 33.4 and 37.1 per cent, respectively. Isolate CST-21 showed significant inhibition in mycelial growth against FOC as 30.0 per cent while, isolate CST-22 showed significant inhibition as 32.8 per cent in case of FOL. In case of volatile assay CST-14 again showed highest inhibitory effect against test pathogens ranged from 29.3 - 39.4 percent respectively. Similarly in case of non-volatile assay, 100 percent inhibition in mycelial growth of test pathogens caused by CST-14 @ 50% concentration. Present investigations concluded that isolates CST-14 (T. longibrachiatum) showed significant inhibitory effect against FOL and FOC. It can be used in the form of bio formulation for effective management of soil borne pathogens.

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