Author(s):

Apalowo OE, Adekola MB, Asaolu FT, Oriyomi OV, Ogunleye GS, Areola JO and OO Babalola

Abstract: The study investigated the inhibitory role of kigelia africana leaf extracts and fractions on 5- Lipoxygenase oxidative enzyme. GC-MS analysis was carried out on the ethyl acette fraction. Fresh leaves of Kigelia africana was extracted with 70% (v/v) ethanol and fractionated with n-hexane, ethyl acetate and butanol. Plant extract and fractions were estimated for total phenol content and flavonoid content using gallic acid and quercetin as standard respectively. Anti-lipoxygenase activity was determined from the extract and fractions at 0.1 mg/ml to 0.5 mg/ml. Fraction which exhibited the most favourable inhibitory potential was subjected to GC-MS fingerprinting to identify the phytoconstituents. The highest total phenolic and total flavonoid content was obtained from the ethyl acetate fraction with values of 22.55±0.55 gallic acid equivalent and 4.63±0.19 quercetin equivalent respectively. Lipoxygenase inhibitory activity of the plant extract and fractions showed that the crude extract, aqueous and ethyl acetate fractions exhibited their highest inhibitory potential at 0.5 mg/ml while n-butanol and n-hexane showed their highest inhibitory effect at 0.2 mg/ml and 0.4 mg/ml respectively. GC-MS analysis of the ethyl acetate fraction revealed the presence of several constituents with anti-inflammatory properties. Kigelia africana leaf extract and fractions could serve as a non-selective redox inhibition of 5-lipoxygenase.

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