Author(s):

Sweety Majumder and Mahbubur Rahman

Abstract: A rapid in vitro multiple shoot regeneration protocol has been developed for Heliotropium indicum Linn., an important medicinal herb. Apical buds and nodal segments of field grown plants were used as explants and cultured on Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of plant growth regulators (PGRs) namely IAA, NAA, BAP and Kn. Maximum number of shoots per explant (6.40±0.08 shoots/shoot apex and 7.16±0.50 shoots/nodal explant) was induced after 30 days of culture in the MS medium supplemented with 1.0 mg/l BAP + 1.0 mg/l NAA. The highest elongation of shoot buds (6.01± 0.13) took place on MS medium fortified with 1.5 mg/l BAP + 0.5 mg/l NAA. In terms of rooting, well developed shoots were excised and implanted individually in rooting medium containing half strength MS fortified with 1.0 mg/l IBA. Regenerated plantlets were hardened, acclimatized and successfully established in field condition. About 85% plantlets survived under field conditions.

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