Author(s):

Hirani Nimisha, Mehta DR, Virendra Singh Shekhawat and Umaretiya VR

Abstract: Genetic diversity among ten genotypes of spine gourd (10 females and 10 males) was analyzed using Randomly Amplified Polymorphic DNA (RAPD) markers. A total of 50 RAPD primers were used, of which 28 primers gave amplification and generated 182 fragments. The numbers of polymorphic bands were 165 (90.66% polymorphism) with an average of 5.89 bands per primer. The Polymorphism Information Content (PIC) for RAPD marker ranged from 0.3598 (OPA-05) to 0.9011 (OPZ-19) with an average value of 0.7555 per primer and RAPD primer index (IPI) differed from 0.7196 (OPA-05) to 9.9121 (OPZ-19) with an average value of 5.1771 per primer. The Jaccard similarity based on RAPD profiles were subjected to UPGMA cluster analysis. The similarity coefficient of cluster analysis ranged from 29.1% to 72.1%. The dendogram generated by RAPD markers revealed two major groups, Cluster-I and Cluster-II. The formation of several sub-clusters with in cluster II suggested the presence of moderate genetic diversity among the 10 spine gourd genotypes of male and female plants. The highest (100%) polymorphi sm percentage was obtained with 23 primers viz., OPA 05, OPA 03, OPC 05, OPD 18, OPD 20, OPL 01, OPL 03, OPZ 11, OPZ 17, OPZ 19, CMN A01, CMN A02, CMN A11, CMN A12, CMN A19, CMN A21, CMN A23, CMN A28, CMN A44, CMN A51, CMN A53, CMN A55 and CMN A57. Therefore, these primers may be most useful for genetic diversity analysis to generate DNA fingerprinting in spine gourd genotypes. However, RAPD primers were unable to distinguish male and female sex determinating plants, which may require another suitable molecular technique.

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