Author(s):

Talari Sneha Latha, Ajmera Rama Rao and Ciddi Veeresham

Abstract: In the present study, an optimized RP-HPLC and HPTLC validated methods was established for quantification of Sennosides, the major constituents of present in selected five marketed Senna herbal formulation with a simple and accuracy. It also carried out dissolution study on Senna herbal tablets and capsules. Sennosides A and B were determined using the external standard calibration method. The solvent impact on the selectivity factor and partition coefficient parameters evaluated and LOD, LOQ were calculated. The reliability of the HPLC and HPTLC methods for analysis of Sennosides A and B was validated through its linearity, reproducibility, repeatability, and recovery. Fina1ly marked herbal formulation standardized by assay of Sennosides A and B through above HPLC and HPTLC validated method. By above method, determination of Sennosides in Senna herbal formulation carried out and the results suggested that all the five selected herbal formulation posses Sennosides with the limits.

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